| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.047956 |
| Chromosome: | chromosome 8 |
| Location: | 3887775 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381900 | (1 of 2) PTHR22763:SF71 - PROTEIN Y105C5B.11, ISOFORM B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGTGGGTGAAGGTAGCCGTTGGTCGGTGAACTGGCTCCGTGCGCTCGG |
| Internal bar code: | CGTTATTATCGGGCCTTACGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 476 |
| LEAP-Seq percent confirming: | 15.7895 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCGTCATCGTCACTACCG |
| Suggested primer 2: | CTTCATCTCCTGCAGCTGCT |