Insertion junction: CLIP2.047964_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.047964
Chromosome:chromosome 10
Location:6624392
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g466050 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAGTAAATAACTCGCTTGCCGTTTCTTCCACGGCAGCAGACCGCTGAC
Internal bar code:ATCAAGGTACGCTGTGCTTTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:3042
LEAP-Seq percent confirming:98.4375
LEAP-Seq n confirming:63
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:64

Suggested primers for confirmation by PCR

Suggested primer 1:GACAAAGTCTGTCGCAGGGA
Suggested primer 2:ATAGCTTCCCGCAACTTGCT