| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047972 |
| Chromosome: | chromosome 14 |
| Location: | 944591 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613550 | HSP70H,FAP203 | Heat shock protein 70H; (1 of 1) PTHR31516:SF11 - PROTEIN H13N06.7-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGTGGCCACAACATCGACCCCTCCCAGTACGCGCGCCACACGGGCCGC |
| Internal bar code: | GCGCAAGTCTCCGGTAAGCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 783 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGAATGGAAGGTGGGTGG |
| Suggested primer 2: | CAAACGCTGCAACTTCGGAA |