Insertion junction: CLIP2.047981_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.047981
Chromosome:chromosome 6
Location:2320380
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g267550 BBS5 Bardet-Biedl syndrome-5 associated protein; (1 of 1) K16748 - Bardet-Biedl syndrome 5 protein (BBS5) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCATCGTTTAAAGCAACCTTCCGTGGCACAACCCAAACTGACACAATCT
Internal bar code:GTAGATCTCTTCGTGCACGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:650
LEAP-Seq percent confirming:79.1667
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTGGTCAGCACGTACAGG
Suggested primer 2:ATGGCTGACCTCTTTGGAGC