| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047990 |
| Chromosome: | chromosome 10 |
| Location: | 938450 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424350 | (1 of 1) PTHR24209:SF7 - PROTEIN DA1-RELATED 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCGATGTACGAATTGCTGGCAAATGCGGTTTGGGTGCACGGCATGGGG |
| Internal bar code: | GTTTAAACGGTATTGCGTGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1953 |
| LEAP-Seq percent confirming: | 87.7778 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 90 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCCTGGGCTGCTAGATGG |
| Suggested primer 2: | CACATCACTAGGCCCTTGCA |