| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.048004 |
| Chromosome: | chromosome 3 |
| Location: | 774542 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146107 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGCGCCGCGTAGCCAGGCACAGCTACTTCTGAAGCATTTTTGCCGCGC |
| Internal bar code: | TCCGAGGAATAAAAGTAAATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1590 |
| LEAP-Seq percent confirming: | 87.1795 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTATCTGTCCAGAGCGAC |
| Suggested primer 2: | GGCTGTGCTGAGTTCTGAGT |