| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.048011 |
| Chromosome: | chromosome 10 |
| Location: | 3035593 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440850 | GPX4 | Glutathione peroxidase 4; (1 of 2) 1.11.1.12 - Phospholipid-hydroperoxide glutathione peroxidase / Peroxidation-inhibiting protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTGCCCCTGCCTTGCCTTCTCCCCCCCATATCCGCAGAAAATCCTGGT |
| Internal bar code: | ACTCTATCCACATGGTTGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 492 |
| LEAP-Seq percent confirming: | 16.6667 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACACTCTGTGCGTGGTAC |
| Suggested primer 2: | TGCGTGCACTCAGATCTTGT |