| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.048019 |
| Chromosome: | chromosome 12 |
| Location: | 6149483 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g535400 | (1 of 1) IPR000104//IPR003590//IPR006553 - Antifreeze protein, type I // Leucine-rich repeat, ribonuclease inhibitor subtype // Leucine-rich repeat, cysteine-containing subtype | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGTTCGTCGTCTGAACCGAGCCGAATGCCGCCTTGTCTCATTGCTCC |
| Internal bar code: | AGCGTACTTCTTAGGTATCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 602 |
| LEAP-Seq percent confirming: | 56.25 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGCTGGAGAACCGTATCG |
| Suggested primer 2: | TTAGGCTCTTCAAGGGCTGC |