Insertion junction: CLIP2.048055_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.048055
Chromosome:chromosome 6
Location:3519706
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278119 (1 of 26) IPR029058//IPR029059 - Alpha/Beta hydrolase fold // Alpha/beta hydrolase fold-5 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAAGCTGACGCCCCAGGCCGCCTCCAGCTCCGCCACCAGCGAGTCAGC
Internal bar code:GCTTGGGATTTTTATGAAGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:4658
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:87
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:87

Suggested primers for confirmation by PCR

Suggested primer 1:ACAAACTCACAAAGCGCGAC
Suggested primer 2:GCGGTTGTAGTGCAAGGTTG