Insertion junction: CLIP2.048097_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.048097
Chromosome:chromosome 11
Location:1365236
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467711 (1 of 3) PTHR30612 - SECA INNER MEMBRANE COMPONENT OF SEC PROTEIN SECRETION SYSTEM intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACACACACACACACACACACGCACACACACACACAAATTCACACACA
Internal bar code:CGGGAGCGATGTACTGGCAAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1019
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ATAGTTCGGGCTGCACACAA
Suggested primer 2:GACCAGATGACTAAGGCGCC