Insertion junction: CLIP2.048101_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.048101
Chromosome:chromosome 12
Location:3599035
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g512950 CIT1 (1 of 1) K07240 - chromate transporter (chrA); Chromate ion transporter intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACACACACACACACACACACACACACACACACACACACACACACACA
Internal bar code:TAGACTAGTTTGTACAATTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2640
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTTCTTGACTGTGCCGAT
Suggested primer 2:CCACACACACACACACACAC