Insertion junction: CLIP2.048116_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.048116
Chromosome:chromosome 1
Location:3910337
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g800074 (1 of 39) PTHR13078//PTHR13078:SF53 - FAMILY NOT NAMED // PROTEIN MAOC-1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCCCGCAGCGCATGAATCGCTTCCGCTGGCGGCGCGGCGCCGCTGTAG
Internal bar code:GGAACTCAGTGTCATGAGCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1871
LEAP-Seq percent confirming:19.0476
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:34
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:AACCAGCACCCATTCCTAGC
Suggested primer 2:GCTAGGAATGGGTGCTGGTT