Insertion junction: CLIP2.048125_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.048125
Chromosome:chromosome 16
Location:4438746
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre16.g668150 (1 of 31) IPR003590 - Leucine-rich repeat, ribonuclease inhibitor subtype intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCATAGCTCTACCTCAATCTTTGCACAGCTGCCCTCATCTGCCCCGTG
Internal bar code:TGTCCCCCACGAGGGGAAATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:283
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TTGGAGATGTGCCGGAAGTC
Suggested primer 2:GCACCGAGGTAGTTGTGTGA