Insertion junction: CLIP2.048127_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.048127
Chromosome:chromosome 3
Location:4140893
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g172800 (1 of 1) K18753 - butyrate response factor 1 (ZFP36L) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCTGGGACAGCAGCGACGGCGAAAAGAAACCCTGCGTGCCGGCGCCGC
Internal bar code:CGTAAGTGTCAGACGTCGGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2634
LEAP-Seq percent confirming:8.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:46
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:CATCCCTGGACCAACCCATG
Suggested primer 2:CATCGTCCATGCCACTACCC