Insertion junction: CLIP2.048132_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.048132
Chromosome:chromosome 3
Location:5084945
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g180250 IPS1,INO1 (1 of 1) 5.5.1.4 - Inositol-3-phosphate synthase / Myo-inositol-1-phosphate synthase; Myo-inositol-1-phosphate synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTGGCAGGGAGGCGGGGAGTCCCCAGCACCCCACTGCTGCGACGCACA
Internal bar code:GCTACGCAAGATCGGTGAATAC

Confirmation - LEAP-Seq

LEAP-Seq distance:3342
LEAP-Seq percent confirming:97.1429
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:ACCGACTTGAGCTTGGTCTG
Suggested primer 2:CCTACGACTATGCGTCCACC