| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.048137 |
| Chromosome: | chromosome 3 |
| Location: | 1764620 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g153700 | FAP347 | Flagellar Associated Protein 347 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCAAACCAGGACCCAGATCCCGCCAGCCTCCGTGGCGCAACTACCGTA |
| Internal bar code: | TTTGCAAGTCATCATCGCGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 877 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCTTGATGACGGAGAGAG |
| Suggested primer 2: | GACAGCCATCTACGCTACCC |