| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.048147 |
| Chromosome: | chromosome 5 |
| Location: | 2385475 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238374 | (1 of 1) K11975 - E3 ubiquitin-protein ligase RNF144 [EC:6.3.2.19] (RNF144) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGTTATGTCCACACATGTTATGTCCCCGCGTGTCATATCCACACACGC |
| Internal bar code: | AAAACGGGTGCTGTAGGGGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 99 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGGGATGGTGGGAATGG |
| Suggested primer 2: | CACACACCTGCAGTTCGTTG |