Insertion junction: CLIP2.048151_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.048151
Chromosome:chromosome 12
Location:3152172
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTCCCTGACTCGAGGGTGTGCGTTTCCGTGTGTCTGTTTCTGGTGTTG
Internal bar code:ATCTCTGTCTACAATATTAATC

Confirmation - LEAP-Seq

LEAP-Seq distance:3227
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:85
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:85

Suggested primers for confirmation by PCR

Suggested primer 1:ATCAGGTCCCATTCCGAGGA
Suggested primer 2:GTTTCGACCTGCCTGACTGA