| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.048155 |
| Chromosome: | chromosome 12 |
| Location: | 3475532 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497200 | FAP216 | EF-Hand Flagellar Associated Protein 216 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTATGGAACGTGCCCATTGGTACAGATGCCTGGAGGACCTGTACGAGAA |
| Internal bar code: | GGTGTTTGTTTGGTTAAGCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2737 |
| LEAP-Seq percent confirming: | 98.5075 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTTGTTTGTCGCCCCAGC |
| Suggested primer 2: | CAACCGAAACAAGCGTCAGG |