Insertion junction: CLIP2.048169_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.048169
Chromosome:chromosome 17
Location:3142917
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g720700 ANK14 Predicted protein with ankyrin repeats; (1 of 7) PF12796//PF13637 - Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_4) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGAAAGTAACCCCCTACACACTGTCACATACTCGCGCTTCCAGGAGGG
Internal bar code:GGAATGCTGAGGTCTATGTTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:339
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CATTGCTCACGTTGGGGTTG
Suggested primer 2:GCCTAAACACGACCCCTTGA