| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.048181 |
| Chromosome: | chromosome 7 |
| Location: | 5008590 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g347400 | (1 of 2) K11135 - Pin2-interacting protein X1 (PINX1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGAGGCACGTCATGGCACGCACACGCAAGCGCAGCAAGAGCAAGCACA |
| Internal bar code: | AGTGCTGGGTATTACTGTTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3791 |
| LEAP-Seq percent confirming: | 27.2 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 91 |
| LEAP-Seq n unique pos: | 125 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGTGGTTACCAGGGCGAG |
| Suggested primer 2: | CGACGAGGAGGATGAAGAGC |