Insertion junction: CLIP2.048184_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.048184
Chromosome:chromosome 5
Location:3082433
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre24.g755597 Chlorophyll a /Pheophorbide a oxygenase-related protein; (1 of 2) 1.14.13.122 - Chlorophyllide-a oxygenase / Cholorophyll-b synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACAGCACCACAACCATACACGTCACTCCACTCCATTCCCACTCATCC
Internal bar code:ACTCCCTGGGCCCATATGTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:181
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GCATGTCGTTGATGATGGGC
Suggested primer 2:GGCAAGGTTGAAGCATGCAA