| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.048187 |
| Chromosome: | chromosome 12 |
| Location: | 4099810 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g801401 | (1 of 6) 2.7.7.49 - RNA-directed DNA polymerase / Revertase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCAACCATAGCGCCTCCTTGGTCGCAAATGATGCCGCCATGTACTCT |
| Internal bar code: | AGGGTGATGGACAGTTCAGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6214 |
| LEAP-Seq percent confirming: | 98.374 |
| LEAP-Seq n confirming: | 121 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 123 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGAAATTCGGCATGACGC |
| Suggested primer 2: | ATACGTCTCGCTACGCACAG |