| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.048203 |
| Chromosome: | chromosome 16 |
| Location: | 2652341 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661450 | HTV3 | (1 of 35) K11253 - histone H3 (H3); Histone H3 variant | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGGACCAGCGGCTGACCAGCCTCCTCGCGCAGGCTCGGCCAGGGCGA |
| Internal bar code: | GCTATAGAACTCTCTCGTTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1002 |
| LEAP-Seq percent confirming: | 42.8571 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACAACGGTCTGAGCAGTC |
| Suggested primer 2: | CAGGGTACGAGTTGTTGGCT |