| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.048212 |
| Chromosome: | chromosome 16 |
| Location: | 7299088 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678437 | CYP97A6 | Cytochrome P450, CYP97 superfamily; (1 of 2) K15747 - beta-ring hydroxylase (LUT5, CYP97A3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACACGCTGATGAAGAGGTCGGTGCCGGCCGCCACCACGTGGCCGCCA |
| Internal bar code: | AGCCTCTTCTAGCAGATTGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 122 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGGCACCATCTTTGCAAC |
| Suggested primer 2: | AGGCCGTTGGAAGTGTTGAT |