| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.048218 |
| Chromosome: | chromosome 5 |
| Location: | 3022500 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g800571 | (1 of 1) IPR007730 - Sporulation-related domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGGCTGAAAGGAAGACCTCAAGCGGGCGGGGTTCTCGAACAAGCATGC |
| Internal bar code: | GTACCCATCTACAAGATGCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4062 |
| LEAP-Seq percent confirming: | 40.5405 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTACGGTGCCATGACCTT |
| Suggested primer 2: | TTTTCGAACAAGCAGGCTGC |