Insertion junction: CLIP2.048219_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.048219
Chromosome:chromosome 7
Location:3920100
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g339300 (1 of 3) PF05180 - DNL zinc finger (zf-DNL) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTGGCACGGCACGCCCGCGTGGACTCCTGCGCGGTTTCGCGCTCCTCC
Internal bar code:AACGTAATCGATAAGGGCACCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1308
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GGTAGGGTTTCTTGAGGGGC
Suggested primer 2:GGAGGTCTGGTACGTCTCCT