| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.048236 |
| Chromosome: | chromosome 14 |
| Location: | 3428768 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630150 | (1 of 1) PTHR10131//PTHR10131:SF52 - TNF RECEPTOR ASSOCIATED FACTOR // TNF RECEPTOR-ASSOCIATED FACTOR FAMILY PROTEIN DDB_G0268444-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTCGTCGTCCGTGTCATCCGTGATCGTGTCATCGTAGTCGTTGACATC |
| Internal bar code: | CCCCCGCCCCCCTGCCCCTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1245 |
| LEAP-Seq percent confirming: | 47.8261 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTGTGTATGCGCATGAG |
| Suggested primer 2: | CAGTGACCACCACCAGACC |