| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.048237 |
| Chromosome: | chromosome 2 |
| Location: | 7844136 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146150 | (1 of 4) PF05066 - HB1, ASXL, restriction endonuclease HTH domain (HARE-HTH) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGATCCGGGTGCGGGCCTGCTGGGGGCGCTAGCCGCTGCAGCTGAAGC |
| Internal bar code: | CAAGCATTATTCTGAGCAGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1862 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTACGATGCTGCTGTGT |
| Suggested primer 2: | TGCCTCCCTGTGTTAGCAAG |