| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.048245 |
| Chromosome: | chromosome 14 |
| Location: | 285992 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g609450 | PWR4 | (1 of 1) IPR000104//IPR006502 - Antifreeze protein, type I // Protein of unknown function PDDEXK-like; PWR motif protein | 3'UTR |
| Cre14.g609500 | PWR5 | PWR motif protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGCCCTTGGATCCTGAAGGCATGGTGGGCGTTTGGGTTGGGTGCAGG |
| Internal bar code: | AATTGGCTAAAAGTCTGTGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 260 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCACAGTGTACACATCCT |
| Suggested primer 2: | GCTCACAATGACCTTGCTGC |