| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.048264 |
| Chromosome: | chromosome 10 |
| Location: | 5148800 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g455551 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCTGGATCAAGGGGGCTGGGTGTCAAGGGTGACTGCTGGGATCAGCG |
| Internal bar code: | TCCTGTTTGACTTTGACCTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1373 |
| LEAP-Seq percent confirming: | 64.2857 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACACCAAACAAGGCGAT |
| Suggested primer 2: | GCGATACGGTAGTTCGCAGA |