Insertion junction: CLIP2.048270_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.048270
Chromosome:chromosome 12
Location:666982
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g493250 FAP358 (1 of 2) PTHR24353//PTHR24353:SF37 - CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE // PROTEIN KINASE DC1-RELATED; Flagellar Associated Protein 358 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGGTTAGGGCCCAACCCGACTTAAGTAACGTGGGCACCCGATTCGCAC
Internal bar code:GCTTCGCTAGACTCTCAGACCT

Confirmation - LEAP-Seq

LEAP-Seq distance:3387
LEAP-Seq percent confirming:98.9899
LEAP-Seq n confirming:98
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:99

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGCTTGGTTGCTGCATCG
Suggested primer 2:AGCAGGCTGGTGTCTAGGTA