| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.048273 |
| Chromosome: | chromosome 11 |
| Location: | 187206 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467547 | TSX1,GTS1:GLTX | Glutamyl/glutaminyl-tRNA synthetase, chloroplast precursor; (1 of 2) 6.1.1.17 - Glutamate--tRNA ligase / Glutamyl-tRNA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATGGCTCCCCATCCCCAGTACCACCGGCACATGCACGCACACACACAC |
| Internal bar code: | ATGGGAGGATTAAGGTACATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3314 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTAGTACTGCTCCTCGCG |
| Suggested primer 2: | CTTCGTCCGCAGTCTTCAGT |