Insertion junction: CLIP2.048275_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.048275
Chromosome:chromosome 2
Location:805850
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g078550 FAP210 (1 of 5) PF13868 - Trichohyalin-plectin-homology domain (TPH); Flagellar Associated Protein 210 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGCTGGGAAACGGGGGCGGTAGGCTAGGGTTGAGGGAGGGCAATGCTG
Internal bar code:TCTATTACGAATATGAATCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:406
LEAP-Seq percent confirming:58.8235
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTACTCCTCGATGCACTC
Suggested primer 2:TGGGGTAGTAGAGGTGAGGC