Insertion junction: CLIP2.048286_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.048286
Chromosome:chromosome 11
Location:3379970
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g477000 SNRK2D snRK1 family in Chlamydomonas, subgroup 2; (1 of 41) IPR000719//IPR011009 - Protein kinase domain // Protein kinase-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCAGGAGCATCCGTATAGCAGATGCGTGGGGAGGGGGTTGTAAATACCA
Internal bar code:TTGGCGAGCTCTTGTCATTCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:101
LEAP-Seq percent confirming:23.5294
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CGCACCAGCAACACATTCAA
Suggested primer 2:CGTCCAAGATGCGCTGAATG