Insertion junction: CLIP2.048302_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.048302
Chromosome:chromosome 12
Location:4013279
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g515950 (1 of 35) IPR001763 - Rhodanese-like domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGAAGCTTCAAGGATACACTCGACTTTCCAATTGCATCGCCGCACAAC
Internal bar code:GTGTGTGTGCCTTGATTAGCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1282
LEAP-Seq percent confirming:43.4783
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTCCATCTTCAACCCCA
Suggested primer 2:TGCACTTAGAAGGCCCACAG