| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.048305 |
| Chromosome: | chromosome 11 |
| Location: | 767966 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467630 | AMI2 | putative amidase; (1 of 2) 3.5.1.4 - Amidase / Acylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCAGGGTGCGTGGGGCGCTTTGGCTGAGCGCGTGGGTGGGCGCTGGTG |
| Internal bar code: | GATGGGTCATTCATTATACTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 452 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACGGCGACAAACACATTG |
| Suggested primer 2: | TGTGACTACGGTTTCCACGG |