| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.048306 |
| Chromosome: | chromosome 6 |
| Location: | 127380 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g249900 | FAP75 | (1 of 1) PTHR23359//PTHR23359:SF81 - NUCLEOTIDE KINASE // ADENYLATE KINASE 8; Flagellar Associated Protein 75 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGCCGGTGGCGACACCCGACACGGAGTGCCCCGCGGCCTGGTGTGCAC |
| Internal bar code: | GTACGGGCGAGTACCTCGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1816 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGTCCTCACTTCACTGGC |
| Suggested primer 2: | AAAGGAGCAAAGGTCGAGGG |