| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.048308 |
| Chromosome: | chromosome 9 |
| Location: | 493486 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749047 | (1 of 3) 2.4.1.68 - Glycoprotein 6-alpha-L-fucosyltransferase / Guanosine diphosphofucose-glycoprotein fucosyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTGCACCTGCTTCTGGACTGCACAGCCCGGCGCGTATGTGGGCAACC |
| Internal bar code: | TAACAAACTATTGTCGCTCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 949 |
| LEAP-Seq percent confirming: | 63.3333 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGCTCCAGTGTTCATCG |
| Suggested primer 2: | CCTCGGTTATATGCAGCGGT |