Insertion junction: CLIP2.048310_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.048310
Chromosome:chromosome 7
Location:4717237
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g345300 UAP1 UDP-N-acetylglucosamine-pyrophosphorylase-related protein; (1 of 1) K00972 - UDP-N-acetylglucosamine/UDP-N-acetylgalactosamine diphosphorylase (UAP1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCGCCCCCATGCACCAAGCGCGGACTCACCATCGCAGGGGCTTGACAT
Internal bar code:GTTAGGCATAGGGAGGGCCAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1754
LEAP-Seq percent confirming:51.3514
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:AACGTCCGTCCAGCACATAG
Suggested primer 2:GGGCAACTTGCTTCACTTGG