Insertion junction: CLIP2.048322_4

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.048322
Chromosome:chromosome 11
Location:1846825
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467350 ACO2 Acyl-CoA oxidase/dehydrogenase; (1 of 1) PF01756//PF02770 - Acyl-CoA oxidase (ACOX) // Acyl-CoA dehydrogenase, middle domain (Acyl-CoA_dh_M) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCACAACTGCTGTGCACTCGCCTCCCCCACAACTGCTGTGCCTCTCCA
Internal bar code:GCGAGGAGTCGGTAGGAATGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:419
LEAP-Seq percent confirming:41.6667
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:GGAAGGGGTTCTCACGGAAG
Suggested primer 2:AGCCGCTGAGGTTCCTTAAC