Insertion junction: CLIP2.048326_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.048326
Chromosome:chromosome 1
Location:1733653
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g009575 (1 of 11) IPR001471//IPR016177//IPR031112 - AP2/ERF domain // DNA-binding domain // AP2-like ethylene-responsive transcription factor intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGTTGGTGCTTGGGGAAAGGATGGAGCCTGCGATGCCCACGGAATGAA
Internal bar code:TACCTATACATGCATCGTCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2280
LEAP-Seq percent confirming:75.7576
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:66

Suggested primers for confirmation by PCR

Suggested primer 1:GTCTCGCTACCGCTACTCAC
Suggested primer 2:CTTGTTGTGTCCAGTGCAGC