| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.048330 |
| Chromosome: | chromosome 16 |
| Location: | 6561546 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674150 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCCACCGGAGAGCAATAATAAACACGATACGTACGGGCAAGATGTAG |
| Internal bar code: | TTAGATGGTTAGTATTTTCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2351 |
| LEAP-Seq percent confirming: | 43.1034 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCTTTCATGTGCAGCCC |
| Suggested primer 2: | TGCGTGCACAGTAGCTGTTA |