| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.048332 |
| Chromosome: | chromosome 6 |
| Location: | 7778811 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g303150 | PTK4 | (1 of 55) PTHR23257//PTHR23257:SF394 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED; Putative tyrosine kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGACGGCTGCTGGGGCTTGGATGAGCGGCGGTGCAGTGACTGCGACAGC |
| Internal bar code: | ATAACGGTGTGACACTCTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2057 |
| LEAP-Seq percent confirming: | 24.5902 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTCCAACACGACCATCGG |
| Suggested primer 2: | CTGGTCCGGATCAGATGTGG |