Insertion junction: CLIP2.048332_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.048332
Chromosome:chromosome 7
Location:4589745
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTGGATAGCGACTGACCAACATGTCCGCGCAGCGGCACGATCGGCATC
Internal bar code:ATGGATGTTTCTCCGGGTTAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1395
LEAP-Seq percent confirming:95.082
LEAP-Seq n confirming:58
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:61

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCAACATTGACCGTTTGT
Suggested primer 2:TCTTTTCGGCACCTGTCCTC