| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.048344 |
| Chromosome: | chromosome 3 |
| Location: | 689409 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145807 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGTCGTGACCTCGCCTAGGCCGTGGGGCAGCTCCAGCTGTCGACTGAC |
| Internal bar code: | AATTGAATCGACATGTTCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2573 |
| LEAP-Seq percent confirming: | 99.1071 |
| LEAP-Seq n confirming: | 111 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 112 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCAAACGTGACTTCAACC |
| Suggested primer 2: | CAACGAAACACACCAGGCAG |