Insertion junction: CLIP2.048345_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.048345
Chromosome:chromosome 10
Location:2062250
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g432650 (1 of 1) 3.6.1.59 - 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA] diphosphatase / M(7)GpppX pyrophosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCTTGCTGTAGATGTCATTCACAAAGTCCAGCTGCAGCTCCAGACCCG
Internal bar code:CTTATTTAGGCCTGCTTTTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1997
LEAP-Seq percent confirming:61.5385
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTCAAGACCCGCTCAACA
Suggested primer 2:AGATCAGTCGCTCCACCTCT