Insertion junction: CLIP2.048347_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.048347
Chromosome:chromosome 17
Location:868579
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g702351 HLM26 putative N-methyltransferase; (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGACGGTGCCGCCGCCGCCCCAGCCGCTGGAACGGCACCAGCAGCTGCC
Internal bar code:TACGGATTTAATGAAGACGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:413
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTGCGTTGTGTTCATTCC
Suggested primer 2:CCACCAACAGTACCCTCACC