Insertion junction: CLIP2.048354_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.048354
Chromosome:chromosome 2
Location:517654
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g076650 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAGTGAGACATGAGCATGCATGTGCGCGTGTCGCTCAGTTGTCCCGTTG
Internal bar code:TCCTATCAGAACCAGCACCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1231
LEAP-Seq percent confirming:31.25
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TCGAACACGCAGACTCGAAA
Suggested primer 2:CCCTCCCCACATCATATGCC