| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.048356 |
| Chromosome: | chromosome 3 |
| Location: | 8280683 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199500 | FAP129 | Flagellar Associated Protein 129 | intron |
| Cre03.g199550 | (1 of 1) IPR000504//IPR012677//IPR014720 - RNA recognition motif domain // Nucleotide-binding alpha-beta plait domain // Double-stranded RNA-binding domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGAACTTCAAACTGGCTGCCAGGACGTTCCCCTTCCAAACGCTATCGC |
| Internal bar code: | GCATATGTTCGCCAGTCTTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2061 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATACCCAAGGTGGCACCAT |
| Suggested primer 2: | GTCCACGCTCTTGACTGTCA |